Isolation and Expression Analysis of <i>BvNHX1</i> from <i>Beta vulgaris</i> with High Sucrose

In present study, the vacuolar Na + /H  exchanger gene was isolated by homologous cloning technology from the high-sucrose  Beta vulgaris  (‘BS02’), referred as  BvNHX1 , which contained an ORF of 1 659 bp, encoded 552 amino acids, protein molecular weight 61.31 kD, and theoretical isoelectric point 6.31. The protein by   gene had 12 transmembrane domains conserved Nhap, Na_H_Exchanger b_cpa1 superfamily, and grouped with various NHXs Chenopodiaceae plants, such Salicornia europaea ,  Atriplex dimorphostegia Suaeda salsa belonged to Class I in vacuolar  exchanger family. Under 400 mmol/L NaCl, 200 KCl 15 ABA, the expression of   reached peaks leaves roots, respectively,  in significantly higher than that indicating  was induced ABA, it may play a greater role roots response abiotic stresses. This study will lay foundation for salt tolerance mechanism in  vulgaris  with high sucrose, and provide solid reliable basis genetic improvement sucrose.